| 质粒名称: | pENTR ARRET |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 111384 |
| 存储实验室: | Klaus Holzmann |
|
载体骨架基本信息 |
|
| 载体名称: | pENTR/D-hH1 |
| 载体出品公司: | Invitrogen |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 采用与pENTR TERRA（Addgene质粒#111383）相同的策略构建。 |
| 载体类型: | Gateway Entry Cloning |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | ARRET |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 868 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pENTR ARRET was a gift from Klaus Holzmann (Addgene plasmid # 111384 ; http://n2t.net/addgene:111384 ; RRID:Addgene_111384) |
买家导航
pENTRARRET载体基本信息
pENTRARRET载体图谱质粒图谱和多克隆位点信息
pENTRARRET载体简介
pENTRARRET载体序列
LOCUS pENTR ARRET 3470 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION ARRET with approximately 130 telomere hexanucleotide repeats of
TTAGGG and TTGGGG variant sequences.
ACCESSION .
VERSION .
KEYWORDS pENTR ARRET
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3470)
TITLE telomeric repeat-containing RNA (TERRA and ARRET) plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3470)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3470
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind complement(894..993)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(1010..1029)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(1011..1029)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1034..1050)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1034..1050)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1152..1171)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 1163..1972
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(1230..1249)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
rep_origin 2119..2707
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2608..2627
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2861..2878
/label=L4440
/note="L4440 vector, forward primer"
terminator 3037..3064
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 3156..3242
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 3291..3313
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 3305..3322
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3306..3322
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3338..3437
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
ORIGIN
1 cgatgaattc cgaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
61 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
121 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
181 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
241 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
301 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
361 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
421 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
481 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
541 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
601 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
661 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
721 aaccctaacc ctaaccctaa ccccaaccct aaccctaacc ctaacgctaa ccctaacccc
781 aaccctaacc ccaaccccaa ccccaacccc aaccccaacc ggaattccgc ggaattcgag
841 ctcggtaccc ggggatcgac aattccactt tttttaattc tcgagatatc tagacccagc
901 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
961 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
1021 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
1081 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
1141 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
1201 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
1261 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
1321 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
1381 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
1441 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
1501 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
1561 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
1621 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
1681 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
1741 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
1801 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
1861 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
1921 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
1981 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
2041 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
2101 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
2161 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
2221 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
2281 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
2341 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
2401 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
2461 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
2521 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
2581 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
2641 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
2701 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
2761 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
2821 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
2881 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
2941 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac
3001 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
3061 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
3121 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
3181 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
3241 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
3301 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact
3361 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
3421 gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgat cgactggatc
//
